Expressed protein ligation using an N-terminal cysteine containing fragment generated in vivo from a pelB fusion protein
نویسندگان
چکیده
منابع مشابه
Expressed protein ligation
The introduction of noncanonical amino acids and biophysical probes into peptides and proteins, and total or segmental isotopic labelling has the potential to greatly aid thedeterminationofprotein structure, functionandprotein– protein interactions. To obtain a peptide as large as possible by solid-phase peptide synthesis, native chemical ligation was introduced to enable synthesis of proteins ...
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Chemically modified proteins are invaluable tools for studying the molecular details of biological processes, and they also hold great potential as new therapeutic agents. Several methods have been developed for the site-specific modification of proteins, one of the most widely used being expressed protein ligation (EPL) in which a recombinant α-thioester is ligated to an N-terminal Cys-contain...
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ژورنال
عنوان ژورنال: Protein Expression and Purification
سال: 2007
ISSN: 1046-5928
DOI: 10.1016/j.pep.2007.04.002